Application
Immunohistochemistry of ARTS in human lung tissue with ARTS antibody at 2 μg/mL.Application
Western blot analysis of ARTS expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with ARTS antibody at 2 μg /ml.Application
Immunofluorescence of ARTS in Human Lung cells with ARTS antibody at 20 μg/mL.| Product Name | ARTS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ARTS Antibody: H5, ARTS, MART, SEP4, CE5B3, PNUTL2, hucep-7, BRADEION, hCDCREL-2, Septin-4, Apoptosis-related protein in the TGF-beta signaling pathway |
| Product description | ARTS Antibody: Apoptosis is related to many diseases and development. Mitochondrial proteins, such as cytochrome c, Apaf-1, and AIF play important role in apoptosis. A novel mitochondrial septin-like protein was identified recently and designated ARTS for apoptosis related protein in TGF-beta signaling pathway. ARTS that is encoded by the human septin H5/PNUTL2/CDCrel2b gene is located to mitochondria and translocates to the nucleus when apoptosis occurs. ARTS is expressed in many tissues. It enhances cell death induced by TGF-beta and, to a lesser extent, by other apoptotic agents, such as TNF-α and Fas ligand.1) Larisch S, Yi Y, Lotan R, Kerner H, et al. A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif. Nat Cell Biol 2000;2(12):915-21 |
| Immunogen | ARTS antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human ARTS. The immunogen is located within the first 50 amino acids of ARTS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ARTS antibody can be used for detection of ARTS by Western blot at 2 μg/mL. A band at 32 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SEPT4 |
|---|---|
| Alternative Names | ARTS Antibody: H5 ARTS MART SEP4 CE5B3 PNUTL2 hucep-7 BRADEION hCDCREL-2 Septin-4 Apoptosis-related protein in the TGF-beta signaling pathway |
| Molecular Weight(MW) | 32 kDa |
| Sequence Similarities | 4) Cat. No. 10-101 - Human Lung Tissue Slide |
Application
Immunohistochemistry of ARTS in human lung tissue with ARTS antibody at 2 μg/mL.
Application
Western blot analysis of ARTS expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with ARTS antibody at 2 μg /ml.
Application
Immunofluorescence of ARTS in Human Lung cells with ARTS antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1302 - Human Lung Tissue Lysate |
|---|---|
| Application Notes | ARTS antibody can be used for detection of ARTS by Western blot at 2 μg/mL. A band at 32 kDa can be detected. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ARTS antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ARTS Antibody is supplied in PBS containing 0.02% sodium azide. |
