Application
ARV1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in A2058 cell line lysates (35ug/lane).Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: human kidney lysate Lane 3: HL-60 whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing A2058 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IApplication
Overlay histogram showing A2058 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I| Product Name | ARV1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein ARV1, hARV1, ARV1 |
| Product description | May act as a mediator of sterol homeostasis (Potential).1) Tong, F., et al. J. Biol. Chem. 285(44):33632-33641(2010) |
| Immunogen | This ARV1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human ARV1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ARV1 |
|---|---|
| Alternative Names | Protein ARV1 hARV1 ARV1 |
| Molecular Weight(MW) | 31 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
ARV1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in A2058 cell line lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: human kidney lysate Lane 3: HL-60 whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing A2058 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I
Application
Overlay histogram showing A2058 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit I| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
