ARX (Aristaless-related genes), a subset of the Paired-related homeobox genes, is a group of regulators of essential events during vertebrate embryogenesis. Arx was highly conserved between mouse and zebrafish. Studies have disclosed its neuromeric expression in the forebrain and longitudinal expression in the floor plate in mouse and zebrafish. It is also expressed in the dorsal telencephalon (presumptive cerebral cortex) of mouse embryos.1) Hirata, T., et al., (2006) Development 133 (8), 1433-1443.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ARX.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ARX antibody can be used for detection of ARX by ELISA at 1:1562500. ARX antibody can be used for detection of ARX by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Mouse NIH-3T3 at 5.0 ug/ml.
Positive Control
1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Application Notes
ARX antibody can be used for detection of ARX by ELISA at 1:1562500. ARX antibody can be used for detection of ARX by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ARX antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt