Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).| Product Name | ASAP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 1, 130 kDa phosphatidylinositol 4,5-bisphosphate-dependent ARF1 GTPase-activating protein, ADP-ribosylation factor-directed GTPase-activating protein 1, ARF GTPase-activating protein 1, D |
| Product description | Possesses phosphatidylinositol 4,5-biphosphate-dependent GTPase-activating protein activity for ARF1 (ADP ribosylation factor 1) and ARF5 and a lesser activity towards ARF6. May coordinate membrane trafficking with cell growth or actin cytoskeleton remodeling by binding to both SRC and PIP2. May function as a signal transduction protein involved in the differentiation of fibroblasts into adipocytes and possibly other cell types (By similarity).1) Muller, T., et al. Oncogene 29(16):2393-2403(2010) |
| Immunogen | This ASAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 360-388 amino acids from the Central region of human ASAP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASAP1 |
|---|---|
| Alternative Names | Arf-GAP with SH3 domain ANK repeat and PH domain-containing protein 1 130 kDa phosphatidylinositol 4 5-bisphosphate-dependent ARF1 GTPase-activating protein ADP-ribosylation factor-directed GTPase-activating protein 1 ARF GTPase-activating protein 1 D |
| Molecular Weight(MW) | 125 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
