Application
ASAP3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in CHO cell line lysates (35ug/lane).Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Product Name | ASAP3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Arf-GAP with SH3 domain, ANK repeat and PH domain-containing protein 3, Development and differentiation-enhancing factor-like 1, Protein up-regulated in liver cancer 1, ASAP3, DDEFL1, UPLC1 |
| Product description | This gene encodes a member of a subfamily of ADP-ribosylation factor(Arf) GTPase-activating proteins that contain additional ankyrin repeat and pleckstrin homology domains. The Arf GAP domain of this protein catalyzes the hydrolysis of GTP bound to Arf proteins. The encoded protein promotes cell differentiation and migration and has been implicated in cancer cell invasion. Alternative splicing results in multiple transcript variants.1) Ismail, S.A., et al. Cell 141(5):812-821(2010) |
| Immunogen | This ASAP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human ASAP3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HamsterHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASAP3 |
|---|---|
| Alternative Names | Arf-GAP with SH3 domain ANK repeat and PH domain-containing protein 3 Development and differentiation-enhancing factor-like 1 Protein up-regulated in liver cancer 1 ASAP3 DDEFL1 UPLC1 |
| Molecular Weight(MW) | 99 kDa |
Application
ASAP3 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in CHO cell line lysates (35ug/lane).
Application
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
