Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Product Name | ASCL4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASCL4, HASH4, bHLHa44 |
| Product description | ASCL4, a basic helix-loop-helix transcription factor, is essential for the determination of cell fate and the development and differentiation of numerous tissues. It could be a transcriptional regulator involved in skin development.Basic helix-loop-helix transcription factors, such as ASCL4, are essential for the determination of cell fate and the development and differentiation of numerous tissues (Jonsson et al., 2004 [PubMed 15475265]).1) Jonsson, M., (2004) Genomics 84 (5), 859-866. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ASCL4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ASCL4 antibody can be used for detection of ASCL4 by ELISA at 1:312500. ASCL4 antibody can be used for detection of ASCL4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASCL4 |
|---|---|
| Alternative Names | ASCL4 HASH4 bHLHa44 |
| Molecular Weight(MW) | 19 kDa |
Application
Antibody used in WB on Human MCF-7 at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | ASCL4 antibody can be used for detection of ASCL4 by ELISA at 1:312500. ASCL4 antibody can be used for detection of ASCL4 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ASCL4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
