Application
Antibody used in WB on Human at: 1:1000 (Lane1: 10 ug COS-7 cell lysate).Application
Antibody used in WB on Human Liver at 5.0 ug/ml.| Product Name | ASL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ASL, ASAL |
| Product description | ASL is a member of the lyase 1 family. The protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in its gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency.This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.1) Tanaka, T., (2002) Tohoku J. Exp. Med. 198 (2), 119-124. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ASL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ASL antibody can be used for detection of ASL by ELISA at 1:62500. ASL antibody can be used for detection of ASL by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ASL |
|---|---|
| Alternative Names | ASL ASAL |
| Molecular Weight(MW) | 49 kDa, 52 kDa, 52 kDa, 49 kDa |
| Entrez Gene | 435 |
|---|---|
| Protein Accession | NP_001020115 |
Application
Antibody used in WB on Human at: 1:1000 (Lane1: 10 ug COS-7 cell lysate).
Application
Antibody used in WB on Human Liver at 5.0 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | ASL antibody can be used for detection of ASL by ELISA at 1:62500. ASL antibody can be used for detection of ASL by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ASL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
