Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
ASMT Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of ASMT Antibody in 293 cell line lysates (35ug/lane)Application
Western blot analysis of lysate from Y79 cell line, using ASMT Antibody at 1:1000.| Product Name | ASMT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Acetylserotonin O-methyltransferase, Hydroxyindole O-methyltransferase, HIOMT, ASMT |
| Product description | ASMT belongs to the methyltransferase superfamily, and is located in the pseudoautosomal region (PAR) at the end of the short arms of the X and Y chromosomes. The encoded enzyme catalyzes the final reaction in the synthesis of melatonin, and is abundant in the pineal gland.1) Melke, J., et al. Mol. Psychiatry 13(1):90-98(2008) |
| Immunogen | This ASMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 212-241 amino acids from the Central region of human ASMT. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:500-1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ASMT |
|---|---|
| Alternative Names | Acetylserotonin O-methyltransferase Hydroxyindole O-methyltransferase HIOMT ASMT |
| Molecular Weight(MW) | 38 kDa |
Application
Flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
ASMT Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of ASMT Antibody in 293 cell line lysates (35ug/lane)
Application
Western blot analysis of lysate from Y79 cell line, using ASMT Antibody at 1:1000.| Application Notes | For WB starting dilution is: 1:500-1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM271748