Application
Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | ATF1 (phospho Ser63) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATF1, Activating transcription factor 1, Cyclic-AMP-dependent transcription factor ATF-1, TCR-ATF1, TREB36 protein |
| Product description | This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.1) Mackenzie KF, et al. (2013) J Immunol 190, 565-77 |
| Immunogen | ATF1 (phospho Ser63) antibody was raised against a peptide sequence around phosphorylation site of serine 63 (R-P-S (p)-Y-R) derived from Human ATF1 . |
| Modification | p-Ser63 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF1 |
|---|---|
| Alternative Names | ATF1 Activating transcription factor 1 Cyclic-AMP-dependent transcription factor ATF-1 TCR-ATF1 TREB36 protein |
| Molecular Weight(MW) | 29 kDa |
| Tissue Specificity | The antibody detects endogenous level of ATF1 only when phosphorylated at serine 63. |
| Entrez Gene | 466 |
|---|---|
| Protein Accession | NP_005162.1 |
Application
Western blot analysis of extracts from HT29 cells treated with Insulin using Phospho-ATF1 (Ser63) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
