Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.Application
Antibody used in IHC on Human Spleen at 5 ug/ml.| Product Name | ATF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATF1, EWS-ATF1, FUS/ATF-1, TREB36 |
| Product description | ATF1 binds the cAMP response element (CRE) (consensus: 5'-GTGACGT [AC] [AG]-3'), a sequence present in many viral and cellular promoters. ATF1 binds to the Tax-responsive element (TRE) of HTLV-I. ATF1 mediates PKA-induced stimulation of CRE-reporter genes.1) Iwasaki, K., (2007) J. Biol. Chem. 282 (31), 22335-22343. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ATF1 antibody can be used for detection of ATF1 by ELISA at 1:12500. ATF1 antibody can be used for detection of ATF1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF1 |
|---|---|
| Alternative Names | ATF1 EWS-ATF1 FUS/ATF-1 TREB36 |
| Molecular Weight(MW) | 29 kDa |
Application
Antibody used in WB on Human Placenta at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human Spleen at 5 ug/ml.| Positive Control | 1) Cat. No. 1309 - Human Placenta Lysate |
|---|---|
| Application Notes | ATF1 antibody can be used for detection of ATF1 by ELISA at 1:12500. ATF1 antibody can be used for detection of ATF1 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
