Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF4 (Phospho-Ser245).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using ATF4 (Phospho-Ser245).| Product Name | ATF4 (phospho Ser245) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CREB2, TXREB, CREB-2, TAXREB67, EIF4EL1, EIF4F, C/ATF, C/EBP-related ATF, CREB2 |
| Product description | ATF4 encodes a transcription factor that was originally identified as a widely expressed mammalian DNA bindingprotein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.1) Yang X, et al. (2004) . Cell. 117 (3): 387-398. |
| Immunogen | ATF4 (Phospho-Ser245) antibody was raised against a peptide sequence around phosphorylation site of serine 245 (N-R-S (p) -L-P) derived from Human ATF4. |
| Modification | p-Ser245 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF4 |
|---|---|
| Alternative Names | CREB2 TXREB CREB-2 TAXREB67 EIF4EL1 EIF4F C/ATF C/EBP-related ATF CREB2 |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | This antibody detects endogenous level of ATF4 only when phosphorylated at serine 245. |
| Entrez Gene | 468 |
|---|---|
| Protein Accession | NP_001666.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF4 (Phospho-Ser245).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV using ATF4 (Phospho-Ser245).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
