Application
Antibody used in IHC on Human at 5.0 ug/ml.Application
Antibody used in WB on Mouse NIH-3T3 at 1.25 ug/ml.| Product Name | ATF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATF4, Atf-4, C/ATF, CREB2, TAXREB67 |
| Product description | Atf4 binds to asymmetric cAMP response elements (CRE) as a heterodimer and to palindromic CRE's as a homodimer.1) Smith, J.A., et al., (2006) J. Virol. 80 (4), 2019-2033. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse ATF4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ATF4 antibody can be used for detection of ATF4 by ELISA at 1:62500. ATF4 antibody can be used for detection of ATF4 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF4 |
|---|---|
| Alternative Names | ATF4 Atf-4 C/ATF CREB2 TAXREB67 |
| Molecular Weight(MW) | 42 kDa |
Application
Antibody used in IHC on Human at 5.0 ug/ml.
Application
Antibody used in WB on Mouse NIH-3T3 at 1.25 ug/ml.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
|---|---|
| Application Notes | ATF4 antibody can be used for detection of ATF4 by ELISA at 1:62500. ATF4 antibody can be used for detection of ATF4 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATF4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
