Application
Western blot analysis of ATF4 Antibody (S245) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate.Application
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of ATF4 using rabbit anti-ATF4 Antibody (S245) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2).| Product Name | ATF4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Tax-responsive enhancer element B67; ATF4 protein |
| Product description | ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain.1) Gombart,A.F., J. Leukoc. Biol. 81 (6), 1535-1547 (2007) |
| Immunogen | This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-252 amino acids from human ATF4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATF4 |
|---|---|
| Alternative Names | Tax-responsive enhancer element B67 ATF4 protein |
| Molecular Weight(MW) | 39 kDa |
Application
Western blot analysis of ATF4 Antibody (S245) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate.
Application
Western Blot at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of ATF4 using rabbit anti-ATF4 Antibody (S245) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
