Application
Immunofluorescence of ATG101 in mouse liver tissue with ATG101 antibody at 20 μg/mL.Application
Immunohistochemistry of ATG101 in mouse liver with ATG101 antibody at 5 μg/mL.Application
Western blot analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ATG101 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG101 Antibody: C12orf44, C12orf44, PP894, Autophagy-related protein 101 |
| Product description | ATG101 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG101 is a recently discovered protein that stabilizes ATG13, another autophagy protein that forms a complex with the mammalian homologs of ATG1, ULK1 and ULK2, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. ATG101 also interacts with ULK1 and is essential for autophagy.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906. |
| Immunogen | ATG101 antibody was raised against a 16 amino acid synthetic peptide near the center of human ATG101. The immunogen is located within amino acids 80 - 130 of ATG101. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ATG101 antibody can be used for detection of ATG101 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C12orf44 |
|---|---|
| Alternative Names | ATG101 Antibody: C12orf44 C12orf44 PP894 Autophagy-related protein 101 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
| Entrez Gene | 60673 |
|---|---|
| Protein Accession | NP_001092143 |
Application
Immunofluorescence of ATG101 in mouse liver tissue with ATG101 antibody at 20 μg/mL.
Application
Immunohistochemistry of ATG101 in mouse liver with ATG101 antibody at 5 μg/mL.
Application
Western blot analysis of ATG101 in human liver tissue lysate with ATG101 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1304 - Human Liver Tissue Lysate |
|---|---|
| Application Notes | ATG101 antibody can be used for detection of ATG101 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG101 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG101 Antibody is supplied in PBS containing 0.02% sodium azide. |
