Application
Immunofluorescence of ATG16 in Hela cells with ATG16 antibody at 4 μg/mL.Application
Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 μg/mL.Application
Western blot analysis of ATG16 in HeLa cell lysate with ATG16 antibody at (A) 0.25 and (B) 0.5 μg/mL.| Product Name | ATG16 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG16 Antibody: IBD10, WDR30, APG16L, ATG16A, ATG16L, UNQ9393/PRO34307, Autophagy-related protein 16-1, APG16-like 1 |
| Product description | ATG16 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene.2004; 23:2891-906. |
| Immunogen | ATG16 antibody was raised against a 18 amino acid synthetic peptide from near the center of human ATG16. The immunogen is located within amino acids 190 - 240 of ATG16. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG16L1 |
|---|---|
| Alternative Names | ATG16 Antibody: IBD10 WDR30 APG16L ATG16A ATG16L UNQ9393/PRO34307 Autophagy-related protein 16-1 APG16-like 1 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunofluorescence of ATG16 in Hela cells with ATG16 antibody at 4 μg/mL.
Application
Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 μg/mL.
Application
Western blot analysis of ATG16 in HeLa cell lysate with ATG16 antibody at (A) 0.25 and (B) 0.5 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ATG16 antibody can be used for the detection of ATG16 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG16 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG16 Antibody is supplied in PBS containing 0.02% sodium azide. |
