Application
Immunofluorescence of ATG2B in K562 cells with ATG2B antibody at 20 μg/mL.Application
Western blot analysis of ATG2B in K562 cell lysate with ATG2B antibody at (A) 1 and (B) 2 μg/ml| Product Name | ATG2B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG2B Antibody: C14orf103, C14orf103, Autophagy-related protein 2 homolog B |
| Product description | ATG2B Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. Another member of the autophagy family of proteins is ATG2B, one of two homologs of ATG2 that is essential for autophagosome formation and important for regulation of size and distribution of lipid droplets. Relatively high rates of ATG2B mutations were observed in gastric and colorectal carcinomas, suggesting that deregulating the autophagy process may contribute to cancer development.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906. |
| Immunogen | ATG2B antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATG2B. The immunogen is located within amino acids 450 - 500 of ATG2B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| ATG2B antibody can be used for detection of ATG2B by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG2B |
|---|---|
| Alternative Names | ATG2B Antibody: C14orf103 C14orf103 Autophagy-related protein 2 homolog B |
| Molecular Weight(MW) | 229 kDa |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | ATG2B antibody is predicted to not cross-react with other ATG2A. |
Application
Immunofluorescence of ATG2B in K562 cells with ATG2B antibody at 20 μg/mL.
Application
Western blot analysis of ATG2B in K562 cell lysate with ATG2B antibody at (A) 1 and (B) 2 μg/ml| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ATG2B antibody can be used for detection of ATG2B by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG2B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG2B Antibody is supplied in PBS containing 0.02% sodium azide. |
