Application
Immunohistochemistry of ATG4A in human spleen tissue with ATG4A antibody at 5 μg/ml.Application
Western blot analysis of ATG4A in EL4 cell lysate with ATG4A antibody at (A) 1 and (B) 2 μg/ml.| Product Name | ATG4A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Autophagy related 4A cysteine peptidase, APG4-A, APG4-A, AUTL2 |
| Product description | Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4A, also known as AUTL2, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906. |
| Immunogen | ATG4A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4A. The immunogen is located within the last 50 amino acids of ATG4A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ATG4A antibody can be used for detection of ATG4A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG4A |
|---|---|
| Alternative Names | Autophagy related 4A cysteine peptidase APG4-A APG4-A AUTL2 |
| Molecular Weight(MW) | Predicted: 48 kDa Observed: 44 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | ATG4A antibody is human, mouse and rat reactive. At least two isoforms of ATG4A are known to exist; this antibody will detect both isoforms. ATG4A is predicted to not cross-react with other ATG4 proteins. |
Application
Immunohistochemistry of ATG4A in human spleen tissue with ATG4A antibody at 5 μg/ml.
Application
Western blot analysis of ATG4A in EL4 cell lysate with ATG4A antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | ATG4A antibody can be used for detection of ATG4A by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG4A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ATG4A antibody is supplied in PBS containing 0.02% sodium azide. |
