Application
Immunofluorescence of ATG4C in human lung tissue with ATG4C antibody at 20 μg/ml.Application
Immunohistochemistry of ATG4C in human lung tissue with ATG4C antibody at 5 μg/ml.Application
Western blot analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 μg/ml.| Product Name | ATG4C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG4C Antibody: APG4C, AUTL1, AUTL3, APG4-C, APG4C, Cysteine protease ATG4C, AUT-like 3 cysteine endopeptidase |
| Product description | Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4C, also known as autophagin-3, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). Recent studies have shown that ATG4C and Beclin-1 are regulated by the microRNA miR-376b; overexpression of miR-376b led to decreased mRNA and protein levels, thereby blocking starvation and TOR inhibition-related autophagy (4).1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906. |
| Immunogen | ATG4C antibody was raised against an 18 amino acid peptide near the carboxy terminus of human ATG4C. The immunogen is located within the last 50 amino acids of ATG4C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ATG4C antibody can be used for detection of ATG4C by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG4C |
|---|---|
| Alternative Names | ATG4C Antibody: APG4C AUTL1 AUTL3 APG4-C APG4C Cysteine protease ATG4C AUT-like 3 cysteine endopeptidase |
| Molecular Weight(MW) | Predicted: 50 kDa Observed: 57 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | ATG4C antibody is human, mouse and rat reactive. ATG4C is predicted to not cross-react with other ATG4 proteins. |
Application
Immunofluorescence of ATG4C in human lung tissue with ATG4C antibody at 20 μg/ml.
Application
Immunohistochemistry of ATG4C in human lung tissue with ATG4C antibody at 5 μg/ml.
Application
Western blot analysis of ATG4C in A549 cell lysate with ATG4C antibody at 1 μg/ml.| Positive Control | 1) Cat. No. 1203 - A549 Cell Lysate |
|---|---|
| Application Notes | ATG4C antibody can be used for detection of ATG4C by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG4C antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ATG4C antibody is supplied in PBS containing 0.02% sodium azide. |
