Application
Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 μg/ml.Application
Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 μg/ml.Application
Western blot analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 μg/ml.| Product Name | ATG4D Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Autophagy related 4D cysteine peptidase, APG4D, APG4-D, AUTL4 |
| Product description | Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2). ATG4D, also known as AUTL4, is one of four mammalian orthologs of the yeast ATG4 protein; all four are cysteine proteases (3). ATG4 is required for ATG8 conjugation to phosphatidylethanolamine on autophagosomal membranes. In mammals, each ATG4 homolog shows a selective preference for the ATG8 homologs (4).1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene 2004; 23:2891-906. |
| Immunogen | ATG4D antibody was raised against a 17 amino acid peptide near the amino terminus of human ATG4D. The immunogen is located within the first 50 amino acids of ATG4D. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ATG4D antibody can be used for detection of ATG4D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG4D |
|---|---|
| Alternative Names | Autophagy related 4D cysteine peptidase APG4D APG4-D AUTL4 |
| Molecular Weight(MW) | Predicted: 52 kDa Observed: 49 kDa |
| Sequence Similarities | 2) Cat. No. 11-701 - Human Testis Tissue Slide |
| Tissue Specificity | ATG4D antibody is human, mouse and rat reactive. At least two isoforms of ATG4D are known to exist; this antibody will detect only the larger isoform. ATG4D is predicted to not cross-react with other ATG4 proteins. |
Application
Immunofluorescence of ATG4D in human testis tissue with ATG4D antibody at 20 μg/ml.
Application
Immunohistochemistry of ATG4D in human testis tissue with ATG4D antibody at 5 μg/ml.
Application
Western blot analysis of ATG4D in human testis tissue lysate with ATG4D antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1313 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | ATG4D antibody can be used for detection of ATG4D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG4D antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ATG4D antibody is supplied in PBS containing 0.02% sodium azide. |
