Application
Immunofluorescence of ATG5 in Human Spleen cells with ATG5 antibody at 5 μg/mL.Application
Immunohistochemistry of ATG5 in human spleen tissue with ATG5 antibody at 10 μg/mL.Application
Western blot analysis of ATG5 in rat spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 1 μg/mL.| Product Name | ATG5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG5 Antibody: ASP, APG5, APG5L, hAPG5, APG5-LIKE |
| Product description | ATG5 Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12; this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906. |
| Immunogen | ATG5 antibody was raised against a 16 amino acid synthetic peptide from near the amino terminus of human ATG5. The immunogen is located within the first 50 amino acids of ATG5. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| WB:1:500~1:2000 IF:1:100~1:500 IHC-P:1:50~1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG5 |
|---|---|
| Alternative Names | ATG5 Antibody: ASP APG5 APG5L hAPG5 APG5-LIKE |
| Tissue Specificity | Three isoforms of ATG5 are known to exist; this ATG5 antibody will only detect the longest isoform. |
Application
Immunofluorescence of ATG5 in Human Spleen cells with ATG5 antibody at 5 μg/mL.
Application
Immunohistochemistry of ATG5 in human spleen tissue with ATG5 antibody at 10 μg/mL.
Application
Western blot analysis of ATG5 in rat spleen tissue lysate with ATG5 antibody at (A) 1 and (B) 1 μg/mL.| Positive Control | Rat Spleen Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 IF:1:100~1:500 IHC-P:1:50~1:200 |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG5 Antibody is supplied in PBS containing 0.02% sodium azide. |
