Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.Application
Antibody used in WB on Human HepG2 at 1 ug/ml.Application
Antibody used in IHC on Human Placenta.Application
Antibody used in IHC on Human Prostate.| Product Name | ATG5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG5, APG5, APG5L, ASP, hAPG5, APG5-LIKE |
| Product description | ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. ATG5 may play an important role in the apoptotic process, possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.1) Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATG5. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ATG5 antibody can be used for detection of ATG5 by ELISA at 1:1562500. ATG5 antibody can be used for detection of ATG5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG5 |
|---|---|
| Alternative Names | ATG5 APG5 APG5L ASP hAPG5 APG5-LIKE |
| Molecular Weight(MW) | 32 kDa |
Application
Antibody used in WB on Hum. Fetal Liver at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Heart at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Application
Antibody used in IHC on Human Placenta.
Application
Antibody used in IHC on Human Prostate.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ATG5 antibody can be used for detection of ATG5 by ELISA at 1:1562500. ATG5 antibody can be used for detection of ATG5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATG5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
