Application
Immunohistochemistry staining of prostate (paraffin-embedded sections) with anti-human Prostate-specific antigen| Product Name | PSA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [A67 - B / E3] to PSAKallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Its protein product is a protease present in seminal plasma. It is thought to function normally in the liquefaction of seminal coagulum, presumably by hydrolysis of the high molecular mass seminal vesicle protein. Serum level of this protein, called PSA in the clinical setting, is useful in the diagnosis and monitoring of prostatic carcinoma. Alternate splicing of this gene generates two transcript variants encoding different isoforms. Additional transcript variants have been described, but it is unclear if these transcripts are normally expressed or if they are specific to benign or malignant tumors. |
| Immunogen | Tissue / cell preparation (Human). 30 kDa protein fraction prepared from human seminal plasma. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | A67-B/E3 |
| Host Species | Mouse |
| Tested Applications | ELISAIHC-PWB |
| ELISA: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | P07288 APS hK3 354 PSA 176820 KLK2A1 KLK3 |
|---|---|
| Target | PSA |
| Tissue Specificity | This antibody is specific for PSA. |
Application
Immunohistochemistry staining of prostate (paraffin-embedded sections) with anti-human Prostate-specific antigen| Application Notes | ELISA: Use at an assay dependant dilution. IHC-P: Use at an assay dependant dilution. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. WB: Use at an assay dependant dilution. Optimal dilutions/concentrations should be determined by the end user.: |
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
