Application
Immunofluorescence of ATG9A in human heart tissue with ATG9A antibody at 20 μg/mL.Application
Immunohistochemistry of ATG9A in human heart tissue with ATG9A antibody at 5 μg/mL.Application
Western blot analysis of ATG9A in mouse heart tissue lysate with ATG9A antibody at 1 μg/mL.| Product Name | ATG9A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG9A Antibody: mATG9, APG9L1, MGD3208, Autophagy-related protein 9A, APG9-like 1 |
| Product description | ATG9A Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9A, a multi-spanning membrane protein localizing to the Golgi apparatus and late endosomes, has been proposed to mediate membrane transport to generate autophagosomes. ATG9A has also been implicated as a regulator of STING (stimulator of interferon genes)-mediated innate immune response.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906. |
| Immunogen | ATG9A antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ATG9A. The immunogen is located within amino acids 720 - 770 of ATG9A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ATG9A antibody can be used for detection of ATG9A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG9A |
|---|---|
| Alternative Names | ATG9A Antibody: mATG9 APG9L1 MGD3208 Autophagy-related protein 9A APG9-like 1 |
| Sequence Similarities | 2) Cat. No. 10-501 - Human Heart Tissue Slide |
Application
Immunofluorescence of ATG9A in human heart tissue with ATG9A antibody at 20 μg/mL.
Application
Immunohistochemistry of ATG9A in human heart tissue with ATG9A antibody at 5 μg/mL.
Application
Western blot analysis of ATG9A in mouse heart tissue lysate with ATG9A antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1401 - Mouse Heart Tissue Lysate |
|---|---|
| Application Notes | ATG9A antibody can be used for detection of ATG9A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG9A antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG9A Antibody is supplied in PBS containing 0.02% sodium azide. |
