Application
Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 μg/mL.Application
Western blot analysis of ATG9B in HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 μg/mL.| Product Name | ATG9B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATG9B Antibody: SONE, APG9L2, NOS3AS, Autophagy-related protein 9B, APG9-like 2, Protein sONE |
| Product description | ATG9B Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene2004; 23:2891-906. |
| Immunogen | ATG9B antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B. The immunogen is located within amino acids 850 - 900 of ATG9B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ATG9B antibody can be used for detection of ATG9B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATG9B |
|---|---|
| Alternative Names | ATG9B Antibody: SONE APG9L2 NOS3AS Autophagy-related protein 9B APG9-like 2 Protein sONE |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
Application
Immunocytochemistry of ATG9B in HeLa cells with ATG9B antibody at 10 μg/mL.
Application
Western blot analysis of ATG9B in HeLa cell lysate with ATG9B antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of ATG9B in Hela cells with ATG9B antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | ATG9B antibody can be used for detection of ATG9B by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATG9B antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATG9B Antibody is supplied in PBS containing 0.02% sodium azide. |
