Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATLA2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of ATLA2 Antibody in HepG2 cell line lysates (35ug/lane)| Product Name | ATLA2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Atlastin-2, 365-, ADP-ribosylation factor-like protein 6-interacting protein 2, ARL-6-interacting protein 2, Aip-2, ATL2, ARL6IP2 |
| Product description | ATLA2 is GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. This protein play a role in endoplasmic reticulum tubular network biogenesis.1) Hu,J. Cell 138 (3), 549-561 (2009) |
| Immunogen | This ATLA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the Central region of human ATLA2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATL2 |
|---|---|
| Alternative Names | Atlastin-2 365- ADP-ribosylation factor-like protein 6-interacting protein 2 ARL-6-interacting protein 2 Aip-2 ATL2 ARL6IP2 |
| Molecular Weight(MW) | 66 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with ATLA2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of ATLA2 Antibody in HepG2 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
