Application
Immunohistochemistry of ATM in human lymph node tissue with ATM antibody at 2.5 μg/mL.Application
Immunofluorescence of ATM in Human Lymph Node cells with ATM antibody at 10 μg/mL.Application
Western blot analysis of ATM in Daudi whole cell lysate with ATM antibody at (A) 1 and (B) 2 μg/mL.| Product Name | ATM Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATM Antibody: AT1, ATA, ATC, ATD, ATE, ATDC, TEL1, TELO1, Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated |
| Product description | ATM Antibody: DNA double strand breaks represent a major threat to an organism's genome. Eukaryotic cells have developed mechanisms that sense the presence this damage and initiate suitable responses that can include DNA repair, cell cycle delay, and programmed cell death. The ATM (mutated in Ataxia-Telangiectasia) protein kinase is activated following the formation of DNA double strand breaks, phosphorylating p53 and another kinase CHK2. This initiates a signaling cascade leading to the phosphorylation and inhibition of Cdc25, ultimately preventing cell cycle progression. In some cell types, such as the hemapoietic system, this leads to apoptosis instead of cell cycle arrest. Multiple isoforms of ATM are known to exist.1) Cahill D, Connor B, and Carney JP. Mechanisms of eukaryotic DNA double strand break repair. Front. Biosci. 2006 ; 11 :1958-76. |
| Immunogen | ATM antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human ATM. The immunogen is located within amino acids 2970 - 3020 of ATM. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC-P |
| ATM antibody can be used for detection of ATM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATM |
|---|---|
| Alternative Names | ATM Antibody: AT1 ATA ATC ATD ATE ATDC TEL1 TELO1 Serine-protein kinase ATM Ataxia telangiectasia mutated A-T mutated |
| Sequence Similarities | 3) Cat. No. 11-521 - Human Lymphoid Tissue Tissue Slide |
Application
Immunohistochemistry of ATM in human lymph node tissue with ATM antibody at 2.5 μg/mL.
Application
Immunofluorescence of ATM in Human Lymph Node cells with ATM antibody at 10 μg/mL.
Application
Western blot analysis of ATM in Daudi whole cell lysate with ATM antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | ATM antibody can be used for detection of ATM by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATM antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATM Antibody is supplied in PBS containing 0.02% sodium azide. |
