Application
Immunocytochemistry of ATMIN in A431 cells with ATMIN antibody at 5 μg/mL.Application
Western blot analysis of ATMIN in 293 cell lysate with ATMIN antibody at (A) 1 and (B) 2 μg/ml.Application
Immunofluorescence of ATMIN in A431 cells with ATMIN antibody at 2.5 μg/mL.| Product Name | ATMIN Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATMIN Antibody: ASCIZ, ZNF822, KIAA0431, ATM interactor, Zinc finger protein 822, ASCIZ |
| Product description | The ATM/ATR-substrate CHK2-interacting zinc finger protein (ATMIN), also known as ASCIZ, forms DNA damage-induced nuclear foci that contain the DNA repair protein Rad51 (1). ATMIN is also thought to be involved in embryonic development, as an absence of ATMIN causes late-embryonic lethality in mice with a range of organ development defects (2). It also activates the transcription DYNLL1, a light chain of the dynein motor complex and sequence-specific regulator of protein dimerization of numerous targets. DYNLL1 can bind to and inhibit the transcription activation domain of ATMIN, forming a simple dynamic feedback loop for DYNLL1 expression (3).1) McNees CJ, Conlan LA, Tenis N, et al. ASCIZ regulates lesion-specific Rad51 focus formation and apoptosis after methylating DNA damage. EMBO J. 2005; 24:2447-57. |
| Immunogen | ATMIN antibody was raised against a 19 amino acid peptide near the amino terminus of human ATMIN. The immunogen is located within amino acids 200 - 250 of ATMIN. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 2.5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATMIN |
|---|---|
| Alternative Names | ATMIN Antibody: ASCIZ ZNF822 KIAA0431 ATM interactor Zinc finger protein 822 ASCIZ |
| Molecular Weight(MW) | Predicted: 73, 79, 91 kDa Observed: 72 kDa |
| Sequence Similarities | 2) Cat. No. 17-002 - A-431 Cell Slide |
| Tissue Specificity | ATMIN antibody is human, mouse and rat reactive. At least three isoforms of ATMIN are known to exist. |
Application
Immunocytochemistry of ATMIN in A431 cells with ATMIN antibody at 5 μg/mL.
Application
Western blot analysis of ATMIN in 293 cell lysate with ATMIN antibody at (A) 1 and (B) 2 μg/ml.
Application
Immunofluorescence of ATMIN in A431 cells with ATMIN antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
|---|---|
| Application Notes | ATMIN antibody can be used for detection of ATMIN by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunocytochemistry at 5 μg/mL. For Immunoflorescence start at 2.5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATMIN antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | ATMIN Antibody is supplied in PBS containing 0.02% sodium azide. |
