Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of ATP12A Antibody in K562 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | ATP12A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Potassium-transporting ATPase alpha chain 2, Non-gastric H(+)/K(+) ATPase subunit alpha, Proton pump, ATP12A, ATP1AL1 |
| Product description | ATP12A belongs to the family of P-type cation transport ATPases. This protein is a catalytic subunit of the ouabain-sensitive H+/K+ -ATPase that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for potassium absorption in various tissues.1) Li,J., et.al., Kidney Int. 65 (4), 1301-1310 (2004) |
| Immunogen | This ATP12A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 833-859 amino acids from the Central region of human ATP12A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP12A |
|---|---|
| Alternative Names | Potassium-transporting ATPase alpha chain 2 Non-gastric H(+)/K(+) ATPase subunit alpha Proton pump ATP12A ATP1AL1 |
| Molecular Weight(MW) | 116 kDa |
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of ATP12A Antibody in K562 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human skin reacted with ATP12A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
