Application
Antibody used in WB on Human MCF7 at 1 ug/ml.Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Product Name | ATP2A3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP2A3, SERCA3 |
| Product description | ATP2A3 is one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction.This gene encodes one of the SERCA Ca (2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms.1) Korosec, B., (2008) Cancer Genet. Cytogenet. 181 (2), 112-118. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP2A3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ATP2A3 antibody can be used for detection of ATP2A3 by ELISA at 1:312500. ATP2A3 antibody can be used for detection of ATP2A3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP2A3 |
|---|---|
| Alternative Names | ATP2A3 SERCA3 |
| Molecular Weight(MW) | 109 kDa |
Application
Antibody used in WB on Human MCF7 at 1 ug/ml.
Application
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | ATP2A3 antibody can be used for detection of ATP2A3 by ELISA at 1:312500. ATP2A3 antibody can be used for detection of ATP2A3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATP2A3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
