Application
Immunohistochemistry of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 5 μg/mL.Application
Immunofluorescence of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 20 μg/mL.Application
Western blot analysis of ATP2C1 in mouse brain tissue lysate with ATP2C1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | ATP2C1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP2C1 Antibody: HHD, BCPM, PMR1, SPCA1, hSPCA1, ATP2C1A, KIAA1347, PMR1L, HUSSY-28, Calcium-transporting ATPase type 2C member 1, ATPase 2C1 |
| Product description | ATP2C1 Antibody: ATP2C1, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 1, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in this gene cause Hailey-Hailey disease, an autosomal dominant disorder characterized by persistent blisters and erosions of the skin. Unlike the related protein ATP2C2, ATP2C1 is ubiquitously expressed and displays a lower maximal turnover rate for overall Ca2+-ATPase reaction and a higher apparent affinity for cytosolic Ca2+ activation of phosphorylation. Recent evidence suggests that ATP2C1 is a key regulator of insulin-like growth factor receptor (IGF1R) processing in tumor progression in basal breast cancers.1) Hu Z, Bonifas JM, Beech J, et al. Mutations in ATP2C1, encoding a calcium pump, cause Hailey-Hailey disease. Nat. Genet. 2000; 24:61-5 |
| Immunogen | ATP2C1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human ATP2C1. The immunogen is located within the last 50 amino acids of ATP2C1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| ATP2C1 antibody can be used for detection of ATP2C1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP2C1 |
|---|---|
| Alternative Names | ATP2C1 Antibody: HHD BCPM PMR1 SPCA1 hSPCA1 ATP2C1A KIAA1347 PMR1L HUSSY-28 Calcium-transporting ATPase type 2C member 1 ATPase 2C1 |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (94%) |
| Tissue Specificity | At least four isoforms of ATP2C1 are known to exist; this antibody will recognize only the three longest isoforms. ATP2C1 antibody will not cross-react with ATP2C2. |
| Entrez Gene | 27032 |
|---|---|
| Protein Accession | NP_001001486 |
Application
Immunohistochemistry of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 5 μg/mL.
Application
Immunofluorescence of ATP2C1 in mouse brain tissue with ATP2C1 antibody at 20 μg/mL.
Application
Western blot analysis of ATP2C1 in mouse brain tissue lysate with ATP2C1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1403 - Mouse Brain Tissue Lysate |
|---|---|
| Application Notes | ATP2C1 antibody can be used for detection of ATP2C1 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATP2C1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATP2C1 Antibody is supplied in PBS containing 0.02% sodium azide. |
