Application
Immunofluorescence of ATP2C2 in 3T3 cells with ATP2C2 antibody at 20 μg/mL.Application
Western blot analysis of ATP2C2 in 3T3 cell lysate with ATP2C2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | ATP2C2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | ATP2C2 Antibody: ATP2C2, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 2, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in the related gene ATP2C1 cause Hailey-Hailey disease, for which ATP2C2 does not compensate, suggesting that ATP2C2 plays other physiological roles. Unlike ATP2C1, ATP2C2 has a much more restricted expression pattern and displays a higher maximal turnover rate for overall Ca2+-ATPase reaction and a lower apparent affinity for cytosolic Ca2+ activation of phosphorylation. Overexpression of ATP2C2 in mammary tumors result a Ca2+ influx via the store-operated Ca2+ channel ORAI1 and independent of the STIM1 and STIM2 sensors.1) Xiang M, Mohamalawari D, and Rao R. A novel isoform of the secretory pathway Ca2+,Mn(2+)-ATPase, hSPCA2, has unusual properties and is expressed in the brain. J. Biol. Chem. 2005; 280:11608-14. |
| Immunogen | ATP2C2 antibody was raised against a 19 amino acid synthetic peptide near the amino terminus of human ATP2C2. The immunogen is located within the first 50 amino acids of ATP2C2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| ATP2C2 antibody can be used for detection of ATP2C2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP2C2 |
|---|---|
| Alternative Names | ATP2C2 Antibody: SPCA2 KIAA0703 SPCA2 Calcium-transporting ATPase type 2C member 2 ATPase 2C2 |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | At least three isoforms of ATP2C2 are known to exist. ATP2C2 antibody will not cross-react with ATP2C1 |
Application
Immunofluorescence of ATP2C2 in 3T3 cells with ATP2C2 antibody at 20 μg/mL.
Application
Western blot analysis of ATP2C2 in 3T3 cell lysate with ATP2C2 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1282 - 3T3 (NIH) Cell Lysate |
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| Application Notes | ATP2C2 antibody can be used for detection of ATP2C2 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | ATP2C2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATP2C2 Antibody is supplied in PBS containing 0.02% sodium azide. |
