Application
Western blot analysis of ATP4B Antibody in mouse heart tissue lysates (35ug/lane)Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | ATP4B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Potassium-transporting ATPase subunit beta, Gastric H(+)/K(+) ATPase subunit beta, Proton pump beta chain, ATP4B |
| Product description | ATP4B belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase.1) Bab-Dinitz, E., et al. Biochemistry 48(36):8684-8691(2009) Knouff, C.W., et al. Pharmacogenet. Genomics 18(12):1051-1057(2008) Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005) |
| Immunogen | This ATP4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 52-78 amino acids from the N-terminal region of human ATP4B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP4B |
|---|---|
| Alternative Names | Potassium-transporting ATPase subunit beta Gastric H(+)/K(+) ATPase subunit beta Proton pump beta chain ATP4B |
| Molecular Weight(MW) | 33 kDa |
Application
Western blot analysis of ATP4B Antibody in mouse heart tissue lysates (35ug/lane)
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
