WB
Untransfected: Control 293 Lysate (A) and Human H+/K+ATPase β Transfection: Western blot analysis of H+/K+ATPase β expression in HEK293 (B) whole cell lysate.IHC
Immunoperoxidase staining of human gastric tissue fixed in formalin and embedded in paraffin using H+/K+ATPase β antibody reveals cytoplasmic staining of gastric parietal lobe cells.IHC
HnRNP A1 antibody: Immunoperoxidase staining was performed on formalin fixed and paraffin embedded human ovarian tissue, displaying nuclear staining of follicular cells, ovarian stromal cells, and oocytes.ICC/IF
Immunofluorescence staining of methanol fixed HeLa cells revealed membrane localization.| Product Name | Mouse Monoclonal antibody to H+/K+ ATPase β |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | raised against a peptide mapping within an extracellular domain of H+/K+ ATPase b of human origin. |
| Clonality | monoclonal |
|---|---|
| Isotype | IgG2b,k |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP4B |
|---|---|
| Gene Synonyms | ATP6B |
| Gene Full Name | ATPase H+/K+ transporting subunit beta |
| Gene Summary | The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 33kDa |
WB
Untransfected: Control 293 Lysate (A) and Human H+/K+ATPase β Transfection: Western blot analysis of H+/K+ATPase β expression in HEK293 (B) whole cell lysate.
IHC
Immunoperoxidase staining of human gastric tissue fixed in formalin and embedded in paraffin using H+/K+ATPase β antibody reveals cytoplasmic staining of gastric parietal lobe cells.
IHC
HnRNP A1 antibody: Immunoperoxidase staining was performed on formalin fixed and paraffin embedded human ovarian tissue, displaying nuclear staining of follicular cells, ovarian stromal cells, and oocytes.
ICC/IF
Immunofluorescence staining of methanol fixed HeLa cells revealed membrane localization.| Application Notes | WB:1:100-1:1000 IHC:1:50-1:500 ICC/IF:1:50-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required. |
| Storage Buffer | Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
