Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).| Product Name | ATP6V0C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | V-type proton ATPase 16 kDa proteolipid subunit, V-ATPase 16 kDa proteolipid subunit, Vacuolar proton pump 16 kDa proteolipid subunit, ATP6V0C, ATP6C, ATP6L, ATPL |
| Product description | ATP6V0C is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. ATP6V0C encodes the V0 subunit c.1) O'Callaghan, K.M., et al. J. Biol. Chem. 285(1):381-391(2010) |
| Immunogen | This ATP6V0C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-126 amino acids from the C-terminal region of human ATP6V0C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP6V0C |
|---|---|
| Alternative Names | V-type proton ATPase 16 kDa proteolipid subunit V-ATPase 16 kDa proteolipid subunit Vacuolar proton pump 16 kDa proteolipid subunit ATP6V0C ATP6C ATP6L ATPL |
| Molecular Weight(MW) | 16 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: C. Elegans |
Application
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
