ATP6V0D2 is the subunit of the integral membrane V0 complex of vacuolar ATPase. Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells, thus providing most of the energy required for transport processes in the vacuolar system. ATP6V0D2 may play a role in coupling of proton transport and ATP hydrolysis.1) Nishi, T. (2003) Biol. Cell 95 (7), 453-457.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP6V0D2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ATP6V0D2 antibody can be used for detection of ATP6V0D2 by ELISA at 1:62500. ATP6V0D2 antibody can be used for detection of ATP6V0D2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 0.2-1 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
ATP6V0D2 antibody can be used for detection of ATP6V0D2 by ELISA at 1:62500. ATP6V0D2 antibody can be used for detection of ATP6V0D2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATP6V0D2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.