Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | ATP6V1F Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | V-type proton ATPase subunit F, V-ATPase subunit F, V-ATPase 14 kDa subunit, Vacuolar proton pump subunit F, ATP6V1F, ATP6S14, VATF |
| Product description | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is the V1 domain F subunit protein.1) Supino, R., et al. Ann. N. Y. Acad. Sci. 1171, 606-616 (2009) : |
| Immunogen | This ATP6V1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-111 amino acids from the C-terminal region of human ATP6V1F. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP6V1F |
|---|---|
| Alternative Names | V-type proton ATPase subunit F V-ATPase subunit F V-ATPase 14 kDa subunit Vacuolar proton pump subunit F ATP6V1F ATP6S14 VATF |
| Molecular Weight(MW) | 13 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
