Application
Antibody used in WB on HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human HepG2 at 0.5 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | ATP7A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATP7A, MK, MNK, DSMAX, SMAX3 |
| Product description | The ATP7A gene encodes the Menkes copper-translocating P-type ATPase, a ubiquitous protein that regulates the absorption of copper in the gastrointestinal tract. Inside cells, this protein has a dual function: it delivers copper to cuproenzymes in the Golgi compartment and effluxes excess copper. The trafficking mechanism and catalytic activity combine to facilitate absorption and intercellular transport of copper. Menkes disease, a systemic copper deficiency disorder, is caused by mutations in the ATP7A gene. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ATP7A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| ATP7A antibody can be used for detection of ATP7A by ELISA at 1:312500. ATP7A antibody can be used for detection of ATP7A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ATP7A |
|---|---|
| Alternative Names | ATP7A MK MNK DSMAX SMAX3 |
| Molecular Weight(MW) | 30 kDa, 163 kDa |
| Entrez Gene | 538 |
|---|---|
| Protein Accession | NP_000043.3 |
Application
Antibody used in WB on HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human HepG2 at 0.5 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | ATP7A antibody can be used for detection of ATP7A by ELISA at 1:312500. ATP7A antibody can be used for detection of ATP7A by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store ATP7A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
