Application
Immunocytochemistry of ATR in K562 cells with ATR antibody at 2 μg/mL.Application
Immunofluorescence of ATR in K562 cells with ATR antibody at 10 μg/mL.Application
Western blot analysis of ATR in K562 cell lysates with ATR antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Product Name | ATR Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATR Antibody: ATR, GAPO, TEM8, ATR, Anthrax toxin receptor 1, Tumor endothelial marker 8 |
| Product description | ATR Antibody: The Anthrax toxin receptor (ATR) was initially discovered as the tumor endothelial marker 8 (TEM8). This protein, which exists in three isoforms (36, 40, and 60 kDa), is highly expressed in tumor vessels as well as in the vasculature of developing embryos, suggesting that it may normally play a role in angiogenesis. However, it also acts as the receptor for anthrax toxin. Following the binding of this protein by the protective antigen (PA) of anthrax, PA is cleaved and heptamerizes to form the binding site for both edema factor (EF) and lethal factor (LF). This complex is then endocytosed by the cell; acidification in endosomes allows the release of EF and LF into the cytoplasm where they interfere with MAPK signaling and induce apoptosis.1) Carson-Walter EB, Watkins DN, Nanda A, et al. Cell surface tumor endothelial markers are conserved in mice and humans. Can. Res. 2001; 61:6649-6655. |
| Immunogen | ATR antibody was raised against a peptide corresponding to 13 amino acids near the C-terminus of human ATR. The immunogen is located within the last 50 amino acids of ATR. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| ATR antibody can be used for detection of ATR by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANTXR1 |
|---|---|
| Alternative Names | ATR Antibody: ATR GAPO TEM8 ATR Anthrax toxin receptor 1 Tumor endothelial marker 8 |
| Sequence Similarities | 2) Cat. No. 17-004 - K-562 Cell Slide |
| Tissue Specificity | ATR antibody will recognize only the largest isoform. |
Application
Immunocytochemistry of ATR in K562 cells with ATR antibody at 2 μg/mL.
Application
Immunofluorescence of ATR in K562 cells with ATR antibody at 10 μg/mL.
Application
Western blot analysis of ATR in K562 cell lysates with ATR antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | ATR antibody can be used for detection of ATR by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | ATR antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | ATR Antibody is supplied in PBS containing 0.02% sodium azide. |
