Application
Western blot analysis using AURKB monoclonal antibody against truncated AURKB recombinant protein and SKN - SH cell lysate.| Product Name | AURKB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AIK2, AIM1, AIRK2, ARK2, STK1, STK12, STK5, Aurora 1, AIM-1, AIK2, AurB, IPL1, PPP1R48, aurkb-sv1, aurkb-sv2 |
| Product description | AURKB (aurora kinase B, AIK2 or aurora-B), with 344-amino acid protein(about 39 kDa),localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB is a mitotic protein kinase, which phosphorylates histone H3 and regulates Chromosomal segregation during mitosis and meiosis.It may regulates several stages of mitosis such as centrosome separation, chromosome segregation and cytokinesis.Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. ARK-2 transcripts arepresent at high levels in human thymus and fetal liver.ARK-2 protein levels are maximal during both S and G2/M phases1) Song J et al. Immunity 22:621-31 (2005). |
| Immunogen | Ni-NTA purified truncated recombinant AURKB expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | AURKB |
|---|---|
| Alternative Names | AIK2 AIM1 AIRK2 ARK2 STK1 STK12 STK5 Aurora 1 AIM-1 AIK2 AurB IPL1 PPP1R48 aurkb-sv1 aurkb-sv2 |
Application
Western blot analysis using AURKB monoclonal antibody against truncated AURKB recombinant protein and SKN - SH cell lysate.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1:2,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | AURKB monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
