Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Antibody is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3), and Flag-tagged Aurora C (lane 4). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).| Product Name | Aurora-C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aurora kinase C, Aurora 3, Aurora/IPL1-related kinase 3, ARK-3, Aurora-related kinase 3, Aurora/IPL1/Eg2 protein 2, Serine/threonine-protein kinase 13, Serine/threonine-protein kinase aurora-C, AURKC, AIE2, AIK3, AIRK3, ARK3, STK13 |
| Product description | Chromosomal segregation during mitosis as well as meiosis is regulated by kinases and phosphatases. The Aurora kinases, members of the Ser/Thr protein kinase family, associate with microtubules during chromosome movement and segregation. Aurora kinase C may play a part in organizing microtubules in relation to the function of the centrosome/spindle pole during mitosis. This protein is localized to centrosome from anaphase to cytokinesis. Expression is limited to testis in normal cells. Elevated expression levels are seen only in a subset of cancer cells such as HepG2, HuH7 and HeLa cells. Aurora-C expression is maximum at M phase.1) Kimura, M., et al., J. Biol. Chem. 274(11):7334-7340 (1999). |
| Immunogen | This Aurora-C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 115-145 amino acids from the Central region of human Aurora-C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AURKC |
|---|---|
| Alternative Names | Aurora kinase C Aurora 3 Aurora/IPL1-related kinase 3 ARK-3 Aurora-related kinase 3 Aurora/IPL1/Eg2 protein 2 Serine/threonine-protein kinase 13 Serine/threonine-protein kinase aurora-C AURKC AIE2 AIK3 AIRK3 ARK3 STK13 |
| Molecular Weight(MW) | 36 kDa |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Antibody is used in Western blot to detect Aurora C in lysates of 293 cells expressing Flag tag (lane 1), Flag-tagged Aurora A (lane 2), Flag-tagged Aurora B (lane 3), and Flag-tagged Aurora C (lane 4). Data is kindly provided by Drs. K. Sasai and S. Sen from the University of Texas MD Anderson Cancer Center (Houston, TX).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
