Application
Immunohistochemistry of Aven in human spleen tissue with Aven antibody at 5 μg/mL.Application
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 μg/mL.Application
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.| Product Name | Aven Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Aven Antibody: PDCD12, Cell death regulator Aven |
| Product description | Aven Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Aven is a recently discovered protein that blocks apoptosis induced by Apaf-1 and caspase-9. It is thought that Aven functions by binding to Bcl-xL, an antiapoptotic member of the Bcl-2 family, and to Apaf-1, possibly interfering with the ability of Apaf-1 to self-associate, suggesting that Aven impedes Apaf-1-mediated caspase activation. Higher levels of Aven mRNA are seen in patients with acute leukemia than in control patients, suggesting that Aven may be useful as a prognostic indicator in leukemia patients.1) Lockshin RA, Osborne B, and Zakeri Z. Cell death in the third millennium. Cell Death Differ. 2000; 7:2-7. |
| Immunogen | Aven antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human Aven. The immunogen is located within the first 50 amino acids of Aven. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| Aven antibody can be used for detection of Aven by Western blot at 1 μg/mL. Despite its predicted molecular weight, Aven often migrates at 55 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AVEN |
|---|---|
| Alternative Names | Aven Antibody: PDCD12 Cell death regulator Aven |
| Sequence Similarities | 3) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of Aven in human spleen tissue with Aven antibody at 5 μg/mL.
Application
Immunofluorescence of AVEN in Human Spleen cells with AVEN antibody at 20 μg/mL.
Application
Western blot analysis of Aven in Raji cell lysate with Aven antibody at 1 μg/mL in (A) the presence and (B) the absence of blocking peptide.| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | Aven antibody can be used for detection of Aven by Western blot at 1 μg/mL. Despite its predicted molecular weight, Aven often migrates at 55 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aven antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Aven Antibody is supplied in PBS containing 0.02% sodium azide. |
