Application
Western blot analysis of AXIN1 in SK-N-SH cell lysate with AXIN1 antibody at 1 μg/mL.Application
Immunofluorescence of AXIN1 in human brain tissue with AXIN1 antibody at 20 μg/mL.| Product Name | AXIN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | AXIN1 Antibody: AXIN1 is a cytoplasmic protein which contains a regulation of G-protein signaling (RGS) domain and a dishevelled and axin (DIX) domain and is thought to function as a negative regulator of the WNT signaling pathway that regulates embryonic axis formation. AXIN1 interacts with adenomatosis polyposis coli (APC), beta-catenin, glycogen synthase kinase 3 beta, forming a tetrameric complex resulting in the regulation of the stabilization of beta-catenin. Mutations in the AXIN1 gene have been associated various carcinomas, indicating that it also functions as a tumor suppressor.1) Zeng L, Fagotto F, Zhang T, et al. The mouse Fused locus encodes Axin, an inhibitor of the Wnt signaling pathway that regulates embryonic axis formation. Cell1997; 90:181-92. |
| Immunogen | AXIN1 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human AXIN1. The immunogen is located within amino acids 620 - 670 of AXIN1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| AXIN1 antibody can be used for detection of AXIN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | AXIN1 |
|---|---|
| Alternative Names | AXIN1 Antibody: AXIN PPP1R49 AXIN Axin-1 Axis inhibition protein 1 hAxin |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of AXIN1 in SK-N-SH cell lysate with AXIN1 antibody at 1 μg/mL.
Application
Immunofluorescence of AXIN1 in human brain tissue with AXIN1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | AXIN1 antibody can be used for detection of AXIN1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | AXIN1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | AXIN1 Antibody is supplied in PBS containing 0.02% sodium azide. |
