This gene is one of seven beta-1,4-galactosyltransferase
(beta4GalT) genes. They encode type II membrane-bound glycoproteins
that appear to have exclusive specificity for the donor substrate
UDP-galactose; all transfer galactose in a beta1,4 linkage to
similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a
distinct function in the biosynthesis of different glycoconjugates
and saccharide structures. As type II membrane proteins, they have
an N-terminal hydrophobic signal sequence that directs the protein
to the Golgi apparatus and which then remains uncleaved to function
as a transmembrane anchor. By sequence similarity, the beta4GalTs
form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and
beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme
encoded by this gene appears to mainly play a role in glycolipid
biosynthesis. Two alternatively spliced transcript variants have
been found for this gene.1) Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Immunogen
This B4GALT4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-117 amino acids from the Central region of human B4GALT4.
Western blot analysis in CEM cell line lysates (35ug/lane).
Application Notes
For WB starting dilution is: 1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.