Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the detected B4GALT5 protein (arrow).| Product Name | B4GALT5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene is one of seven beta-1,4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1,4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The function of the enzyme encoded by this gene is not clear. This gene was previously designated as B4GALT4 but was renamed to B4GALT5. In the literature it is also referred to as beta4GalT2. [provided by RefSeq].1) Kitayama, K., et al. J. Biol. Chem. 282(41):30085-30096(2007) |
| Immunogen | This B4GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 324-350 amino acids from the C-terminal region of human B4GALT5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | B4GALT5 |
|---|---|
| Alternative Names | Beta-1 4-galactosyltransferase 5 Beta-1 4-GalTase 5 Beta4Gal-T5 b4Gal-T5 241- Beta-1 4-GalT II UDP-Gal:beta-GlcNAc beta-1 4-galactosyltransferase 5 UDP-galactose:beta-N-acetylglucosamine beta-1 4-galactosyltransferase 5 B4GALT5 |
| Molecular Weight(MW) | 45 kDa |
Application
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the detected B4GALT5 protein (arrow).| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
