Application
Western blot analysis of BACE2 in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with BACE2 antibody at 1 μg/mL.Application
Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 μg/mL.| Product Name | BACE2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BACE2 Antibody: Accumulation of the amyloid-beta (Abeta) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated by proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and gamma-sites by proteases. The long-sought beta-secretase was recently identified by several groups independently and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). BACE/Asp2 is a novel transmembrane aspartic protease and co-localizes with APP. A BACE homolog was recently cloned and designated BACE2, Asp1, DRAP (for Down region aspartic protease), and memapsin 1. BACE2 also cleaves APP at b-site and at a different site within Abeta. BACE2 locates on chromosome 21q22.3, the so-called ‘Down critical region', suggesting that BACE2 and Abeta may also contribute to the pathogenesis of Down syndrome.1) Vassar R, et al. β-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE. Science 1999;286:735-41 |
| Immunogen | BACE2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human BACE2. The immunogen is located within the last 50 amino acids of BACE2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| BACE2 antibody can be used for detection of BACE2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BACE2 |
|---|---|
| Alternative Names | BACE2 Antibody: ASP1 BAE2 DRAP AEPLC ALP56 ASP21 CDA13 CEAP1 UNQ418/PRO852 Beta-secretase 2 Aspartic-like protease 56 kDa ASP1 |
| Sequence Similarities | 2) Cat. No. 1461 - Rat Heart Tissue Lysate |
Application
Western blot analysis of BACE2 in human heart tissue lysate in the absence (A) or presence (B) of blocking peptide with BACE2 antibody at 1 μg/mL.
Application
Immunohistochemistry of BACE2 in rat heart tissue with BACE2 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1301 - Human Heart Tissue Lysate |
|---|---|
| Application Notes | BACE2 antibody can be used for detection of BACE2 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BACE2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BACE2 Antibody is supplied in PBS containing 0.02% sodium azide. |
