Application
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of BAHD1 Antibody in MDA-MB231 cell line lysates (35ug/lane)| Product Name | BAHD1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAHD1 is a heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. It may act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. It represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins.1) Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009). |
| Immunogen | This BAHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 750-777 amino acids from the C-terminal region of human BAHD1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACS,IHC-P,WB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAHD1 |
|---|---|
| Alternative Names | Bromo adjacent homology domain-containing 1 protein BAH domain-containing protein 1 BAHD1 KIAA0945 |
| Molecular Weight(MW) | 85 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of BAHD1 Antibody in MDA-MB231 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
