Application
Immunohistochemistry of BAMBI in human lung tissue with BAMBI antibody at 5 μg/ml.Application
Western blot analysis of BAMBI in mouse lung tissue lysate with BAMBI antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of BAMBI in human lung tissue with BAMBI antibody at 20 μg/ml.| Product Name | BAMBI Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAMBI (BMP and activin membrane-bound inhibitor homolog, NMA) is a membrane spanning glycoprotein that acts as a negative regulator of TGF-beta signaling during development (1). BAMBI transcription regulation is under the influence of beta-catenin, BMP, smad3 and smad4 (2,3). BAMBI expression can increase in colorectal and hepatocellular carcinomas relative to non-cancerous tissues (4). BAMBI is expressed at high levels during odontogenesis. BAMBI is coexpressed with Bmp-4 during early Xenopus embryogenesis and can be detected in poorly metastatic human melanoma cell lines (5).1) Onichtchouk D, Chen YG, Dosch R, et al. Silencing of TGF-beta signalling by the pseudoreceptor BAMBI. Nature 1999; 401:480-5. |
| Immunogen | BAMBI antibody was raised against a 17 amino acid peptide near the center of human BAMBI. The immunogen is located within amino acids 100 - 150 of BAMBI. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| BAMBI antibody can be used for detection of BAMBI by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BAMBI |
|---|---|
| Alternative Names | BMP and activin membrane-bound inhibitor homolog |
| Molecular Weight(MW) | Predicted: 29 kDa Observed: 26 kDa |
| Sequence Similarities | 2) Cat. No. 10-101 - Human Lung Tissue Slide |
| Tissue Specificity | BAMBI antibody is human, mouse and rat reactive. |
Application
Immunohistochemistry of BAMBI in human lung tissue with BAMBI antibody at 5 μg/ml.
Application
Western blot analysis of BAMBI in mouse lung tissue lysate with BAMBI antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of BAMBI in human lung tissue with BAMBI antibody at 20 μg/ml.| Positive Control | 1) Cat. No. 1402 - Mouse Lung Tissue Lysate |
|---|---|
| Application Notes | BAMBI antibody can be used for detection of BAMBI by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BAMBI antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | BAMBI antibody is supplied in PBS containing 0.02% sodium azide. |
