Application
Western blot analysis of BANF1 in human kidney tissue lysate with BANF1 antibody at (A) 0.5 and (B) 1 μg/mL.Application
Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 μg/mL.| Product Name | BANF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BANF1 Antibody: Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BANF1 also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BANF1 will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BANF1 is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.1) Furukawa K. LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction. J. Cell Sci. 1999; 112:2485-92. |
| Immunogen | BANF1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BANF1. The immunogen is located within the last 50 amino acids of BANF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| BANF1 antibody can be used for detection of BANF1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 20 μg/mL. | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BANF1 |
|---|---|
| Alternative Names | BANF1 Antibody: BAF NGPS BCRP1 D14S1460 BAF BCRG1 Barrier-to-autointegration factor Breakpoint cluster region protein 1 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
Application
Western blot analysis of BANF1 in human kidney tissue lysate with BANF1 antibody at (A) 0.5 and (B) 1 μg/mL.
Application
Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1305 - Human Kidney Tissue Lysate |
|---|---|
| Application Notes | BANF1 antibody can be used for detection of BANF1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BANF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BANF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
