Application
Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 μg/mL.Application
Western blot analysis of BANF1 in Hela cell lysate with BANF1 antibody at 1 μg/mL.| Product Name | BANF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BANF1 Antibody: Barrier-to-autointegration factor 1 (BANF1) is a conserved chromatin protein that non-specifically binds double-stranded DNA. BAF also interacts with a family of nuclear proteins that include LAP2, emerin, and MAN1. It is also a host cell component of retroviral pre-integration complexes (PICs), including that of HIV. BAF will bind to p55 Gag (the structural precursor of HIV-1 virions) as well as its cleaved product matrix. In addition to being a host cell component of the PIC, it is thought that BAF is also present at low levels in incoming virions, and thus might contribute to the assembly or activity of HIV-1 PICs through direct binding to matrix as well as DNA.1) Furukawa K. LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction. J. Cell Sci. 1999; 112:2485-92. |
| Immunogen | BANF1 antibody was raised against a 19 amino acid synthetic peptide from near the amino terminus of human BANF1. The immunogen is located within the first 50 amino acids of BANF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| BANF1 antibody can be used for detection of BANF1 by Western blot at 0.5 - 2 μg/mL. Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BANF1 |
|---|---|
| Alternative Names | BANF1 Antibody: BAF NGPS BCRP1 D14S1460 BAF BCRG1 Barrier-to-autointegration factor Breakpoint cluster region protein 1 |
| Sequence Similarities | 2) Cat. No. 10-401 - Human Kidney Tissue Slide |
Application
Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 μg/mL.
Application
Western blot analysis of BANF1 in Hela cell lysate with BANF1 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | BANF1 antibody can be used for detection of BANF1 by Western blot at 0.5 - 2 μg/mL. Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE. Antibody can also be used for immunoflourescence starting at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BANF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BANF1 Antibody is supplied in PBS containing 0.02% sodium azide. |
