Application
Immunohistochemistry of BAP31 in rat lung tissue with BAP31 antibody at 2 μg/mL.Application
Western blot analysis of BAP31 in Ramos cell lysate with BAP31 antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.| Product Name | BAP31 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | BAP31 Antibody: Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. It may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopicially, is also a potent inducer cell death.1) Ng F, Nguyen M, Kwan T, et al. p28 BAP31, a Bcl-2/Bcl-XL- and procaspase-8-associated proteinin the endoplasmic reticulum. J. Cell Biol.1997; 139:327-38. |
| Immunogen | BAP31 antibody was raised against a 17 amino acid synthetic peptide from near the carboxy terminus of human BAP31. The immunogen is located within the last 50 amino acids of BAP31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| BAP31 antibody can be used for the detection of BAP31 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BCAP31 |
|---|---|
| Alternative Names | BAP31 Antibody: CDM DDCH BAP31 6C6-AG DXS1357E B-cell receptor-associated protein 31 6C6-AG tumor-associated antigen BCR-associated protein 31 |
| Sequence Similarities | 2) Cat. No. 1462 - Rat Lung Tissue Lysate |
Application
Immunohistochemistry of BAP31 in rat lung tissue with BAP31 antibody at 2 μg/mL.
Application
Western blot analysis of BAP31 in Ramos cell lysate with BAP31 antibody at (A) 0.5, (B) 1 and (C) 2μg/mL.| Positive Control | 1) Cat. No. 1225 - Ramos Cell Lysate |
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| Application Notes | BAP31 antibody can be used for the detection of BAP31 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | BAP31 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | BAP31 Antibody is supplied in PBS containing 0.02% sodium azide. |
